antibody against mouse gal 1  (Cell Signaling Technology Inc)

Journal: The Journal of Neuroscience

Article Title: Insulin-Like Growth Factor (IGF) Signaling through Type 1 IGF Receptor Plays an Important Role in Remyelination

doi: 10.1523/JNEUROSCI.23-20-07710.2003

Figure Lengend Snippet: Brain-specific Cre-mediated recombination within oligodendrocyte progenitors in heterozygous mice possessing floxed and null Igf1r alleles and also carrying a CaMKIIα-cre transgene. A, Colocalization (yellow) of cre expression and recombination (green, as can be assessed by a recombination sensitive reporter that expresses β-gal on Cre-mediated recombination) within NG2+ and O4+ oligodendrocyte progenitors (red) in a CamKII-α-cre transgenic mouse line at P1. Scale bar, 30 μm. B, Colocalization of IGF1R expression (red) within O4+ oligodendrocyte progenitors (green) isolated from the corpus callosum of adult wild-type (WT) and ΔIgf1r mice. Two hundred O4+ cells isolated from the corpus callosum of three different WT and ΔIgf1r mice were analyzed. Scale bar, 25 μm.

Article Snippet: Tissue sections were dried, rehydrated, blocked with 5% normal goat serum (NGS), and then stained with the mouse IgM monoclonal O4 supernatant (1:5) or the rabbit polyclonal NG2 antibody (1:100; a gift from Bill Stallcup, Scripps Institute, San Diego, CA) to detect oligodendrocyte progenitors and a mouse monoclonal IgG 1 antibody directed against β-gal (1:100; Promega, Madison, WI) to detect Cre-mediated recombination overnight.

Techniques: Expressing, Transgenic Assay, Isolation